Difference between revisions of "Part:BBa K4387000"
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− | PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to sfGFP and NorR. Two corresponding | + | PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to sfGFP and NorR. Two corresponding integration host factors IHF1 and IHF2 binding sites were removed from the promoter PnorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription. |
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Revision as of 20:58, 28 September 2022
Nitric Oxide Sensing Promoter pNorVβ
PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to sfGFP and NorR. Two corresponding integration host factors IHF1 and IHF2 binding sites were removed from the promoter PnorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]