Difference between revisions of "Part:BBa K4152121"

Revision as of 18:39, 28 September 2022

AOX1 promoter+α-factor secretion signal+propeptide+PK_MT4+His-Tag+AOX1 Terminator

This composite part is made up with 6 basic parts: AOX1 promoter, α-factor secretion signal, propeptide, our target protein PK_MT4, the His-tag and AOX1 terminator. These elements make our target protein better expressed and more convenient for subsequent protein detection.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2349
Illegal XbaI site found at 1725
Illegal PstI site found at 970
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2349
Illegal PstI site found at 970
Illegal NotI site found at 2361
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2349
Illegal BamHI site found at 937
Illegal XhoI site found at 1191
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2349
Illegal XbaI site found at 1725
Illegal PstI site found at 970
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2349
Illegal XbaI site found at 1725
Illegal PstI site found at 970
Illegal AgeI site found at 2427
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4152121 short</partinfo>
-This composite part is made up with 6 basic parts: AOX1 promoter, &#945;-factor secretion signal, Propeptide, our target protein WT, the His-tag and AOX1 terminator. These elements make our target protein better expressed and more convenient for subsequent protein detection.
+This composite part is made up with 6 basic parts: AOX1 promoter, α-factor secretion signal, propeptide, our target protein PK_MT4, the His-tag and AOX1 terminator. These elements make our target protein better expressed and more convenient for subsequent protein detection.
 <!-- Add more about the biology of this part here