Difference between revisions of "Part:BBa K4140003"

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Due to its sensitivity to TyrR we use this promoter to regulate the expression of L7Ae under the effect of tyrR and phenylalanine the activity of paroF is optimum for L7Ae expression but in the presence of TyrR and tyrosine the expression of L7Ae is markedly reduced.
 
Due to its sensitivity to TyrR we use this promoter to regulate the expression of L7Ae under the effect of tyrR and phenylalanine the activity of paroF is optimum for L7Ae expression but in the presence of TyrR and tyrosine the expression of L7Ae is markedly reduced.
  
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==Literature Characterization==
 
  
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[[File:T--AFCM-EGYPT--CAS2.PNG|thumb|Right|Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs. ]]
 
 
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Revision as of 09:14, 7 October 2022


ParoF promoter


Part Description

ParoF promoter is a constitutive promoter present in E. Coli which is sensitive to tyrosine and regulated by tyrR protein, it intitiates the synthesis of DAHP (3-deoxy-D-arabino-heptulosonate 7-phosphate synthase) and it is what makes it sensitive to phenylalanine as TyrR & Phenylalanine complex causes inhibition of the transcription of aroF

Usage

Due to its sensitivity to TyrR we use this promoter to regulate the expression of L7Ae under the effect of tyrR and phenylalanine the activity of paroF is optimum for L7Ae expression but in the presence of TyrR and tyrosine the expression of L7Ae is markedly reduced.






























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]