Difference between revisions of "Part:BBa K4152050"

Latest revision as of 11:04, 29 September 2022

AOX1 promoter

AOX1 promoter is the binding site of universal primers for the transcriptional expression of methanol oxidase-1 expressed by AOX1. In our project, AOX1 promoter is located at the 5' end of Protease K gene. On the one hand, when our linear vector is transferred into yeast cells, AOX1 promoter can recombine with AOX1 gene in yeast chromosome. On the other hand, in the presence of methanol, it acts as the promoter of Proteinase K expression to initiate transcription, so as to express our protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 937
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4152050 short</partinfo>
-AOX1 promoter is the binding site of universal primers for the transcriptional expression of methanol oxidase-1 expressed by AOX1. In our project, AOX1 promoter is located at the 5 'end of Protease K gene. On the one hand, when our linear vector is transferred into yeast cells, AOX1 promoter can recombine with AOX1 gene in yeast chromosome. On the other hand, in the presence of methanol, it acts as the promoter of Proteinase K expression to initiate transcription, so as to express our protein.
+AOX1 promoter is the binding site of universal primers for the transcriptional expression of methanol oxidase-1 expressed by AOX1. In our project, AOX1 promoter is located at the 5' end of Protease K gene. On the one hand, when our linear vector is transferred into yeast cells, AOX1 promoter can recombine with AOX1 gene in yeast chromosome. On the other hand, in the presence of methanol, it acts as the promoter of Proteinase K expression to initiate transcription, so as to express our protein.
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