Difference between revisions of "Part:BBa K4140003"
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Ahmed Mattar (Talk | contribs) (→Usage) |
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==Usage== | ==Usage== | ||
| − | + | Due to its sensitivity to TyrR we use this promoter to regulate the expression of L7Ae under the effect of tyrR and phenylalanine the activity of paroF is optimum for L7Ae expression but in the presence of TyrR and tyrosine the expression of L7Ae is markedly reduced. | |
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==Literature Characterization== | ==Literature Characterization== | ||
Revision as of 17:40, 28 September 2022
ParoF promoter
Part Description
ParoF promoter is a constitutive promoter present in E. Coli which is sensitive to tyrosine and regulated by tyrR protein, it intitiates the synthesis of DAHP (3-deoxy-D-arabino-heptulosonate 7-phosphate synthase) and it is what makes it sensitive to phenylalanine as TyrR & Phenylalanine complex causes inhibition of the transcription of aroF
Usage
Due to its sensitivity to TyrR we use this promoter to regulate the expression of L7Ae under the effect of tyrR and phenylalanine the activity of paroF is optimum for L7Ae expression but in the presence of TyrR and tyrosine the expression of L7Ae is markedly reduced.
Literature Characterization
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]