Difference between revisions of "Part:BBa K4256122"

 
 
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<partinfo>BBa_K4256122 short</partinfo>
  
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It is a component of the CcdA/CcdB Type II Toxin-antitoxin (TA) system. It encodes the CcdB poison, which is a potent inhibitor of cell proliferation. In absence of the antitoxin, the CcdB poison traps DNA-gyrase cleavable complexes, inducing breaks into DNA and cell death. As a model system, by ensuring an antidote-toxin ratio greater than one, this mechanism might prevent the harmful effect of CcdB in plasmid-containing bacteria. The CcdA-CcdB toxin-antitoxin system we used is a killing switch that is relatively nontoxic to human and other mammalian cells.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4256122 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4256122 parameters</partinfo>
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Latest revision as of 16:46, 11 October 2022

ccdB

It is a component of the CcdA/CcdB Type II Toxin-antitoxin (TA) system. It encodes the CcdB poison, which is a potent inhibitor of cell proliferation. In absence of the antitoxin, the CcdB poison traps DNA-gyrase cleavable complexes, inducing breaks into DNA and cell death. As a model system, by ensuring an antidote-toxin ratio greater than one, this mechanism might prevent the harmful effect of CcdB in plasmid-containing bacteria. The CcdA-CcdB toxin-antitoxin system we used is a killing switch that is relatively nontoxic to human and other mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 40
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]