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Revision as of 17:35, 28 September 2022

T7 promoter

Part Description

T7 promoter is a dna sequence that can be recognized by T7 RNA polymerase and is mainly used to regulate gene expression of recombinant proteins,all of which allows it to be used in downstream research approaches.It is considered to be a constitutive promoter with high levels of transcription and expression in the presence of T7 RNAP and minimal interaction with bacterial RNAP.

Usage

Because they are not recognized by E. coli RNAP, T7 promoters can generate considerable levels of transcription in E. coli and have the capacity to regulate themselves independently of E. coli promoters. We employ it to regulate TyrR protein expression constitutively in high levelsl

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.

References

Sequence and Features