Difference between revisions of "Part:BBa K4509569:Design"

Revision as of 18:45, 28 September 2022

HORSERADISH PEROXIDASE with constitutive promoter J23100

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 445
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 565
Illegal AgeI site found at 719
Illegal AgeI site found at 1022
1000
COMPATIBLE WITH RFC[1000]

The promoter J23119 is replaced with J23100. This switch in the design will reduce the burden in the cell during over expression of enzymes.

To be added

Revision as of 14:13, 28 September 2022 (view source) Registry (Talk \| contribs)		Revision as of 18:45, 28 September 2022 (view source) Marie JJ (Talk \| contribs) Newer edit →
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	===Design Notes===		===Design Notes===
−	The promoter J23119 is replaced with ~~J23118~~. This switch in the design will reduce the burden in the cell during over expression of enzymes.	+	The promoter J23119 is replaced with J23100. This switch in the design will reduce the burden in the cell during over expression of enzymes.