Difference between revisions of "Part:BBa K4509569"
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| − | The enzyme horseradish peroxidase, found in the roots of horseradish catalyzes the oxidation of various organic substrates by hydrogen peroxide. It is a metalloenzyme with many isoforms and the most studied type is C. The composite part starts with the | + | The enzyme horseradish peroxidase, found in the roots of horseradish catalyzes the oxidation of various organic substrates by hydrogen peroxide. It is a metalloenzyme with many isoforms and the most studied type is C. The composite part starts with the J23100 constitutive promoter, continues with RBS and is followed by the Horseradish Peroxidase coding sequence. The sequence ends with a terminator and a BamH1 restriction site. |
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Revision as of 14:18, 28 September 2022
HORSERADISH PEROXIDASE with constitutive promoter J23100
The enzyme horseradish peroxidase, found in the roots of horseradish catalyzes the oxidation of various organic substrates by hydrogen peroxide. It is a metalloenzyme with many isoforms and the most studied type is C. The composite part starts with the J23100 constitutive promoter, continues with RBS and is followed by the Horseradish Peroxidase coding sequence. The sequence ends with a terminator and a BamH1 restriction site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 445
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 565
Illegal AgeI site found at 719
Illegal AgeI site found at 1022 - 1000COMPATIBLE WITH RFC[1000]