Difference between revisions of "Part:BBa K4152001:Design"
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===Design Notes=== | ===Design Notes=== | ||
To improve the stability of wild-type proteinase K, we designed 2 mutation sites: T16C-N257C. | To improve the stability of wild-type proteinase K, we designed 2 mutation sites: T16C-N257C. | ||
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===Source=== | ===Source=== | ||
Revision as of 16:46, 28 September 2022
PK_MT1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 235
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 235
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 235
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To improve the stability of wild-type proteinase K, we designed 2 mutation sites: T16C-N257C.
Source
We make multiple site mutations on the basis of a serine protease proteinase K from Tritirachium album limber. (PDB ID: 1ic6)