Difference between revisions of "Part:BBa K4329002"
m (→what is it?) |
|||
| Line 7: | Line 7: | ||
CRISPR/dCas9 is a gene editing technology used to inhibit the expression from the targeted sequence inducing DNA to activate its self-repair process. | CRISPR/dCas9 is a gene editing technology used to inhibit the expression from the targeted sequence inducing DNA to activate its self-repair process. | ||
| − | === | + | ===Usage and Biology=== |
This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGTTAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration. | This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGTTAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
| − | + | ||
<!-- --> | <!-- --> | ||
Revision as of 12:55, 28 September 2022
sgRNA for targeting Bifunctional BirA enzyme
What is it?
CRISPR/dCas9 is a gene editing technology used to inhibit the expression from the targeted sequence inducing DNA to activate its self-repair process.
Usage and Biology
This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGTTAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 19
- 1000COMPATIBLE WITH RFC[1000]