Difference between revisions of "Part:BBa K4140011"
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A CAP binding site and the lac operator sequence lacO are present in this lac promoter. Although the lac promoter is not as potent as the tac or trc promoters, it nevertheless provides for decent quantities of foreign protein production in high copy-number vectors. Expression from the lac promoter is activated in the absence of the lac repressor (LacI). | A CAP binding site and the lac operator sequence lacO are present in this lac promoter. Although the lac promoter is not as potent as the tac or trc promoters, it nevertheless provides for decent quantities of foreign protein production in high copy-number vectors. Expression from the lac promoter is activated in the absence of the lac repressor (LacI). | ||
==Usage== | ==Usage== | ||
| − | + | lac promoter contains A CAP binding site and the lac operator sequence lacO,Expression from the lac promoter is activated in the absence of the lac repressor (LacI). | |
| − | + | Although the lac promoter is not as potent as the tac or trc promoters, we use it as a constitutive promoter for the regulation of (Phenylalanine-specific permease) expression. | |
==Literature Characterization== | ==Literature Characterization== | ||
Revision as of 17:53, 28 September 2022
Lac promoter
Part Description
A CAP binding site and the lac operator sequence lacO are present in this lac promoter. Although the lac promoter is not as potent as the tac or trc promoters, it nevertheless provides for decent quantities of foreign protein production in high copy-number vectors. Expression from the lac promoter is activated in the absence of the lac repressor (LacI).
Usage
lac promoter contains A CAP binding site and the lac operator sequence lacO,Expression from the lac promoter is activated in the absence of the lac repressor (LacI). Although the lac promoter is not as potent as the tac or trc promoters, we use it as a constitutive promoter for the regulation of (Phenylalanine-specific permease) expression.
Literature Characterization
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]