Difference between revisions of "Part:BBa K3743020"

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<partinfo>BBa_K4140013 short</partinfo>
 
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==Part Description==
  
 
It is the RNA domain of CRISPR system which formed of two component crRNA that bind to the target sequence and tracrRNA that connect cas protein to the RNA which act as a handle
 
It is the RNA domain of CRISPR system which formed of two component crRNA that bind to the target sequence and tracrRNA that connect cas protein to the RNA which act as a handle
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==Usage==
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==Literature Characterization==
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[[File:T--AFCM-EGYPT--CAS2.PNG|thumb|Right|Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs. ]]
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==References==
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 09:55, 28 September 2022


VSVg-Fusogen


Part Description

It is the RNA domain of CRISPR system which formed of two component crRNA that bind to the target sequence and tracrRNA that connect cas protein to the RNA which act as a handle

Usage

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1396
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1176
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 987
  • 1000
    COMPATIBLE WITH RFC[1000]