Difference between revisions of "Part:BBa K4345008"
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− | NarX | + | The mutant was designed based on a study of Cavicchioli et al., (1995). The histidine on place 399 was replaced by glutamic acid. This allows the NarX mutants to dimerize but blocks it from phosphorylating NarL (the second messenger). |
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Revision as of 16:08, 7 October 2022
NarX H399-E
The mutant was designed based on a study of Cavicchioli et al., (1995). The histidine on place 399 was replaced by glutamic acid. This allows the NarX mutants to dimerize but blocks it from phosphorylating NarL (the second messenger).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 659
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 659
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 260
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 659
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 659
- 1000COMPATIBLE WITH RFC[1000]