Difference between revisions of "Part:BBa K4232002"
Line 8: Line 8:
  
 
[[File:BIT-China-parts-nirb-pcr.jpeg|350px|thumb|left|'''Figure 1''' The results of PCR about nirb]]
 
[[File:BIT-China-parts-nirb-pcr.jpeg|350px|thumb|left|'''Figure 1''' The results of PCR about nirb]]
−
[[File:BIT-China-parts-nirb-expression.jpeg|thumb|400px|right|'''Figure 2'''The expression of nirb at different oxygen concentrations]]
+
[[File:BIT-China-parts-nirb-expression.jpeg|thumb|420px|right|'''Figure 2'''The expression of nirb at different oxygen concentrations]]
  
  

Revision as of 02:34, 28 September 2022


A oxygen-sensitive promoter

This part codes for the promoter nirB. The nirB promoter, known as nitrite reductase (NADH) large subunit, was derived from the E. coli top10 strain (PCR image shown in Figure 1). It can be used as a sensor of intracellular oxygen concentration due to the fact that it is expressed at much higher levels under low oxygen concentration conditions than under normal oxygen concentration (as shown in Figure 2), regardless of the presence of nitrite or nitrate in the environment

The nirB activity is affected by nitrate and its use needs to be considered with or without removing this effect.

Figure 1 The results of PCR about nirb
Figure 2The expression of nirb at different oxygen concentrations
















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 409
    Illegal BglII site found at 1278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1849
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2396
    Illegal SapI.rc site found at 68
    Illegal SapI.rc site found at 2351