Difference between revisions of "Part:BBa K4341003:Experience"
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===Applications of BBa_K4341003=== | ===Applications of BBa_K4341003=== | ||
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| + | == '''Experience by [https://2022.igem.wiki/ohiostate/ 2022 iGEM OhioState] ''' == | ||
| + | Inspired by OhioState 2021, we fused BBa_R0051 to BBa_K4341000 in an attempt to create a very strong promoter for use in our Phinder Phage. We decided to create a fusion of mCherry to BBa_K4341003 to determine its expression profile. Once the fusion of mCherry and BBa_K4341003 was obtained we attempted to ligate into the pET28a backbone (given to us by Dr. David Wood's Lab). However once isolation via gel extraction was done for our backbone and inserts were digested with BamHI and NotI ligation transformations proved fruitless after many attempts and swapping of buffers. Eventually because of time constraints were unable to pursue the problem any further. | ||
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| + | <center><html><img src='https://static.igem.wiki/teams/4341/wiki/engineering/gel.svg' width='480px'></html></center> | ||
| + | <center><html>Figure 1. Gel Electrophoresis Result: Lane 1 rrnB P1; Lane 2 pR; Lane 3 pR-rrnB P1; Lane 4 t5-pR</html></center> | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 12:50, 12 October 2022
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4341003
Experience by 2022 iGEM OhioState
Inspired by OhioState 2021, we fused BBa_R0051 to BBa_K4341000 in an attempt to create a very strong promoter for use in our Phinder Phage. We decided to create a fusion of mCherry to BBa_K4341003 to determine its expression profile. Once the fusion of mCherry and BBa_K4341003 was obtained we attempted to ligate into the pET28a backbone (given to us by Dr. David Wood's Lab). However once isolation via gel extraction was done for our backbone and inserts were digested with BamHI and NotI ligation transformations proved fruitless after many attempts and swapping of buffers. Eventually because of time constraints were unable to pursue the problem any further.
User Reviews
UNIQ6ec31f23c77b221f-partinfo-00000002-QINU UNIQ6ec31f23c77b221f-partinfo-00000003-QINU