Difference between revisions of "Part:BBa K4387999:Design"

 
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===Source===
 
===Source===
  
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Sequence of the HlyB was obtain from the paper: Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0
+
Sequence of the HlyB was obtain from the paper of Ruano-Gallego et al. [1]
  
 
===References===
 
===References===
 +
[1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0

Revision as of 20:46, 27 September 2022


HlyB of the hemolysin A one-step secretion system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1096
    Illegal AgeI site found at 1266
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No codon optimization was necessary. The sequence was taken directly from the reference paper.


Source

Sequence of the HlyB was obtain from the paper of Ruano-Gallego et al. [1]

References

[1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0