Difference between revisions of "Part:BBa K4260111"
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This part encodes an intein mediated biosensor, consisting of a divided AmilCP chromoprotein gene, a mutated intein RecA N-terminal and C-terminal domains and a ESR1 Ligand Binding Domain. The RecA intein comes from Mycobacterium Tuberculosis (Mtu) which has been mutated with the purpose of making it more stable. | This part encodes an intein mediated biosensor, consisting of a divided AmilCP chromoprotein gene, a mutated intein RecA N-terminal and C-terminal domains and a ESR1 Ligand Binding Domain. The RecA intein comes from Mycobacterium Tuberculosis (Mtu) which has been mutated with the purpose of making it more stable. | ||
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[[File:RecA_intein_biosensor_animated.gif|350px|thumb|right|<i><b>Fig.x:</b>RecA intein behavior when an EDC is present.</i>]] | [[File:RecA_intein_biosensor_animated.gif|350px|thumb|right|<i><b>Fig.x:</b>RecA intein behavior when an EDC is present.</i>]] | ||
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Revision as of 17:28, 27 September 2022
RecA mini intein mediated ESR1 protein with OmpA signal peptide, linker and AmilCP.
This part encodes an intein mediated biosensor, consisting of a divided AmilCP chromoprotein gene, a mutated intein RecA N-terminal and C-terminal domains and a ESR1 Ligand Binding Domain. The RecA intein comes from Mycobacterium Tuberculosis (Mtu) which has been mutated with the purpose of making it more stable.
Design
POR LLENAR
Sequence and Features