Difference between revisions of "Part:BBa K4260111"
| Line 5: | Line 5: | ||
This part encodes an intein mediated biosensor, consisting of a divided AmilCP chromoprotein gene, a mutated intein RecA N-terminal and C-terminal domains and a ESR1 Ligand Binding Domain. The RecA intein comes from Mycobacterium Tuberculosis (Mtu) which has been mutated with the purpose of making it more stable. | This part encodes an intein mediated biosensor, consisting of a divided AmilCP chromoprotein gene, a mutated intein RecA N-terminal and C-terminal domains and a ESR1 Ligand Binding Domain. The RecA intein comes from Mycobacterium Tuberculosis (Mtu) which has been mutated with the purpose of making it more stable. | ||
| + | ===Usage and Biology=== | ||
[[File:RecA_intein_biosensor_animated.gif|350px|thumb|right|<i><b>Fig.x:</b>RecA intein behavior when an EDC is present.</i>]] | [[File:RecA_intein_biosensor_animated.gif|350px|thumb|right|<i><b>Fig.x:</b>RecA intein behavior when an EDC is present.</i>]] | ||
<html> <p align="justify"> | <html> <p align="justify"> | ||
POR LLENAR | POR LLENAR | ||
<p align="justify"> | <p align="justify"> | ||
| − | |||
| − | |||
| − | |||
<!-- --> | <!-- --> | ||
Revision as of 17:28, 27 September 2022
RecA mini intein mediated ESR1 protein with OmpA signal peptide, linker and AmilCP.
This part encodes an intein mediated biosensor, consisting of a divided AmilCP chromoprotein gene, a mutated intein RecA N-terminal and C-terminal domains and a ESR1 Ligand Binding Domain. The RecA intein comes from Mycobacterium Tuberculosis (Mtu) which has been mutated with the purpose of making it more stable.
Usage and Biology
POR LLENAR
Sequence and Features