Difference between revisions of "Part:BBa K4195180"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K4195180 short</partinfo>
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===Biology===
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This sequence is a conserved region of toxin gene ''pirB''.<sup>[1]</sup>It’s used as the detection target of RENDR system.<br/>
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'''Ribozyme ENabled Detection of RNA (RENDR)'''<br/>
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RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the ''Tetrahymena thermophila'' ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.<br/>
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<!-- Add more about the biology of this part here
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[[File:T--XMU-China--RENDR.png|400px]]<br/>
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===Usage and Biology===
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'''Fig. 1 Schematic illustration of RENDR.'''<br/>
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===Usage and Design===
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This part is used as the target of the detection system <partinfo>BBa_K4195187</partinfo>、<partinfo>BBa_K4195188</partinfo>、<partinfo>BBa_K4195189</partinfo> and <partinfo>BBa_K4195190</partinfo>. We build the circuit similar as <partinfo>BBa_K4195178</partinfo>. <br/>
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<span class='h3bb'>Sequence and Features</span>
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Reference<br/>
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<partinfo>BBa_K4195180 SequenceAndFeatures</partinfo>
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[1] Lazarte, J., Kim, Y. R., Lee, J. S., Chun, J. H., Kim, S. W., Jung, J. W., Kim, J., Kayansamruaj, P., Thompson, K. D., Kim, H., & Jung, T. S. (2021). Passive Immunization with Recombinant Antibody VLRB-PirA<sup>vp</sup>/PirB<sup>vp</sup>-Enriched Feeds against ''Vibrio parahaemolyticus'' Infection in ''Litopenaeus'' ''vannamei'' Shrimp. ''Vaccines'', 9(1), 55. https://doi.org/10.3390/vaccines9010055
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===Functional Parameters===
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<partinfo>BBa_K4195180 parameters</partinfo>
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Revision as of 15:14, 10 October 2022

Biology

This sequence is a conserved region of toxin gene pirB.[1]It’s used as the detection target of RENDR system.
Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.
T--XMU-China--RENDR.png
Fig. 1 Schematic illustration of RENDR.

Usage and Design

This part is used as the target of the detection system BBa_K4195187、BBa_K4195188、BBa_K4195189 and BBa_K4195190. We build the circuit similar as BBa_K4195178.
Reference
[1] Lazarte, J., Kim, Y. R., Lee, J. S., Chun, J. H., Kim, S. W., Jung, J. W., Kim, J., Kayansamruaj, P., Thompson, K. D., Kim, H., & Jung, T. S. (2021). Passive Immunization with Recombinant Antibody VLRB-PirAvp/PirBvp-Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp. Vaccines, 9(1), 55. https://doi.org/10.3390/vaccines9010055