Difference between revisions of "Part:BBa K4324001:Design"

 
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  - RBS:  
 
  - RBS:  
  
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  - CDS: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - XYL2
+
  - CDS: XYL2 gene from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Taken from the [https://www.uniprot.org/uniprotkb/Q07993/entry Uniprot database].
−
 
+
−
  gene - from Uniprot
+
  
 
  - Terminator:
 
  - Terminator:
  
 
===References===
 
===References===

Revision as of 01:28, 28 September 2022


lacPO-RBS-XDH-T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 700
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 357


Design Notes

The protein sequence was codon-optimised for Escherichia coli through the IDT codon optimisation tool as gBlocks Gene Fragments.



Source

LacPO: 

- RBS: 

- CDS: XYL2 gene from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Taken from the Uniprot database.

- Terminator:

References