Difference between revisions of "Part:BBa K176042:Design"
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===Design Notes=== | ===Design Notes=== | ||
Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design. | Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design. | ||
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Main parameters for picking VR3~VR12: | Main parameters for picking VR3~VR12: | ||
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Max Mispriming With Other VR3~VR12 Sites: 4 bp | Max Mispriming With Other VR3~VR12 Sites: 4 bp | ||
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| + | ===Source=== | ||
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| + | Reverse complement of VR8 (K176052). Picked from random sequences. | ||
===References=== | ===References=== | ||
Latest revision as of 07:49, 13 August 2009
Reverse primer VR8 (K176052) binding site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.
Main parameters for picking VR3~VR12:
Size: 20 bp (N19 + G or C)
Tm: VF2 Tm ±1℃
GC%: 50%
Max Self Complementarity: 3 bp
Max 3' Self Complementarity: 2 bp
Max Pair Complementarity: 3 bp
Max 3' Pair Complementarity: 2 bp
Max 3' Stability: 3.36 kcal/mol
Max Template(pSB1A3) Mispriming: 6 bp
Max Mispriming With Other VR3~VR12 Sites: 4 bp
Source
Reverse complement of VR8 (K176052). Picked from random sequences.