Difference between revisions of "Part:BBa K4259000"

 
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Cup1 is a gene in Saccharomyces cerevisiae that encodes for metallothionein (MT), a metal-binding protein.  
 
Cup1 is a gene in Saccharomyces cerevisiae that encodes for metallothionein (MT), a metal-binding protein.  
  
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Characterization
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===Usage and Biology===
 
Metallothionein plays an important role in intracellular metal distribution and plays an important role in protecting the cell against heavy metal toxicity and oxidative stress. MT has a a high metal binding affinity an due to its role in metal reduction, previous studies have integrated MT in biological platforms to investigate its potential in the production of metal nanoparticles (NPs). Thus, we expressed Cup1 in an engineered Escherichia Coli to produce gold and silver NPs.  
 
Metallothionein plays an important role in intracellular metal distribution and plays an important role in protecting the cell against heavy metal toxicity and oxidative stress. MT has a a high metal binding affinity an due to its role in metal reduction, previous studies have integrated MT in biological platforms to investigate its potential in the production of metal nanoparticles (NPs). Thus, we expressed Cup1 in an engineered Escherichia Coli to produce gold and silver NPs.  
  
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Design  
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===Design===
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We want to express the Cup1 gene in E. coli, it was therefore codon optimized. Then two overhang sequences with the restriction enzymes BamHI and NdeI were designed and added to the sequence. This part is therefore compatible with vectors that have those two restriction sites. In the Binanox project, the vector pET-16b was used.  
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We codon optimized the sequence for E.coli. Then two overhang sequences with the restriction enzymes BamHI and NdeI were designed and added to the sequence. This part is therefore compatible with vectors that have those two restriction sites. In the Binanox project, the vector pET-16b was used.  
  
  
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4259000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4259000 SequenceAndFeatures</partinfo>

Revision as of 16:27, 26 September 2022


Cup1 for E. coli

Cup1 is a gene in Saccharomyces cerevisiae that encodes for metallothionein (MT), a metal-binding protein.


Usage and Biology

Metallothionein plays an important role in intracellular metal distribution and plays an important role in protecting the cell against heavy metal toxicity and oxidative stress. MT has a a high metal binding affinity an due to its role in metal reduction, previous studies have integrated MT in biological platforms to investigate its potential in the production of metal nanoparticles (NPs). Thus, we expressed Cup1 in an engineered Escherichia Coli to produce gold and silver NPs.

Design

We codon optimized the sequence for E.coli. Then two overhang sequences with the restriction enzymes BamHI and NdeI were designed and added to the sequence. This part is therefore compatible with vectors that have those two restriction sites. In the Binanox project, the vector pET-16b was used.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 123
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]