Difference between revisions of "Part:BBa K4263001"

P_ACC1

Introduction

ACC1 is the major member of ACCs in mammalian^[1].

Characterization

In order to test the function of P_ACC1, we construct "P_ACC1-EGFP-terminator" (Figure 1). If P_ACC1 is functional, we can test the fluorescence intensity of EGFP in supernatant samples obtained from the culture of recombinant P.pastoris GS115 strain.

Figure 1 Gene circuit of P_ACC1-EGFP-terminator

Our results matched the general expected trend (Figure 2). After fermentation experiment in BMMY medium containing 1% methanol. The fluorescence intensity of the samples of recombinant P.pastoris GS115 containing the EGFP gene gradually increased over time . At the same time, we measured the growth curve of the strains.

Figure 2 Fluorescence intensity and OD600 absorbance of samples obtained at different time points from the culture of corresponding recombinant P.pastoris GS115 containing EGFP gene.

Reference

[1] Wang Y, Yu W, Li S, et al. Acetyl-CoA Carboxylases and Diseases[J]. Front Oncol, 2022,12:836058.