Difference between revisions of "Part:BBa K4220000"

(→‎The design and improvement of RTAC:)
(→‎The design and improvement of RTAC:)
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==The design and improvement of RTAC:==
 
==The design and improvement of RTAC:==
 
1.Better interaction with 4 RSPOs--changing the length of linker:
 
1.Better interaction with 4 RSPOs--changing the length of linker:
 +
 
[[File:rtac1.jpg|550px|options|caption]]
 
[[File:rtac1.jpg|550px|options|caption]]
  

Revision as of 12:55, 26 September 2022


FZD5 sp-V5-RTAC


Introduction:

RTAC, Rspo-Targeting Anti-cancer Chimeric protein which can be expressed in mammalian cells and collected in culture medium.

Composition:

ZNRF3+linker+LGR4+human IgGH3 Fc

The design and improvement of RTAC:

1.Better interaction with 4 RSPOs--changing the length of linker:

caption

2.Better secretion capability--changing the type of signaling peptide

3.Better stability--adding an IGg Fc tag


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1254
    Illegal BamHI site found at 2171
    Illegal XhoI site found at 2162
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2274
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 124
    Illegal BsaI site found at 1451
    Illegal BsaI.rc site found at 1139