Difference between revisions of "Part:BBa K4220000"
(→The design and improvement of RTAC:) |
(→The design and improvement of RTAC:) |
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ZNRF3+linker+LGR4+human IgGH3 Fc | ZNRF3+linker+LGR4+human IgGH3 Fc | ||
==The design and improvement of RTAC:== | ==The design and improvement of RTAC:== | ||
| − | Better interaction with 4 RSPOs--changing the length of linker | + | 1.Better interaction with 4 RSPOs--changing the length of linker: |
| + | [[File:rtac1.jpg|options|caption]] | ||
| − | Better secretion capability--changing the type of signaling peptide | + | 2.Better secretion capability--changing the type of signaling peptide |
| − | Better stability--adding an IGg Fc tag | + | 3.Better stability--adding an IGg Fc tag |
Revision as of 12:52, 26 September 2022
FZD5 sp-V5-RTAC
Introduction:
RTAC, Rspo-Targeting Anti-cancer Chimeric protein which can be expressed in mammalian cells and collected in culture medium.
Composition:
ZNRF3+linker+LGR4+human IgGH3 Fc
The design and improvement of RTAC:
1.Better interaction with 4 RSPOs--changing the length of linker:
2.Better secretion capability--changing the type of signaling peptide
3.Better stability--adding an IGg Fc tag
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 70
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1254
Illegal BamHI site found at 2171
Illegal XhoI site found at 2162 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2274
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 124
Illegal BsaI site found at 1451
Illegal BsaI.rc site found at 1139