Difference between revisions of "Part:BBa K4276008"
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Properties: tool for monitor the antigens expression | Properties: tool for monitor the antigens expression | ||
| − | == Usage and biology == | + | === Usage and biology === |
The researches indicate that the major capsid protein vp1 is the main component of the polyomavirus capsid and the main antigens of EV71. vp1 monomers are typically about 350 amino acids in length and can self-assemble into icosahedral structures consisting of 72 pentamers of 360 vp1 molecules. A capsid protein, vp7, is the main antigens of RV, early studies using sera from hyperimmunized animals in cross-neutralization assays described some different serotypes that were found to infect humans and animals based on the vp7 protein. GLBP is a membrane protein belonging to the family of ATP-binding cassette transport proteins. It is widely expressed in the cell wall of wild-type Bifidobacteria and actively transports specific substances across the cell membrane of all organisms using the energy of adenosine triphosphate (ATP). Antigens expressed in fusion with GLBP can induce mucosal immunity and immune memory. Green fluorescent protein (GFP) is a fluorescent tag that facilitates the detection of vp7-vp1 localization on Bifidobacteria. | The researches indicate that the major capsid protein vp1 is the main component of the polyomavirus capsid and the main antigens of EV71. vp1 monomers are typically about 350 amino acids in length and can self-assemble into icosahedral structures consisting of 72 pentamers of 360 vp1 molecules. A capsid protein, vp7, is the main antigens of RV, early studies using sera from hyperimmunized animals in cross-neutralization assays described some different serotypes that were found to infect humans and animals based on the vp7 protein. GLBP is a membrane protein belonging to the family of ATP-binding cassette transport proteins. It is widely expressed in the cell wall of wild-type Bifidobacteria and actively transports specific substances across the cell membrane of all organisms using the energy of adenosine triphosphate (ATP). Antigens expressed in fusion with GLBP can induce mucosal immunity and immune memory. Green fluorescent protein (GFP) is a fluorescent tag that facilitates the detection of vp7-vp1 localization on Bifidobacteria. | ||
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Properties: a protein anchored in the membrane of gram-positive bacteria | Properties: a protein anchored in the membrane of gram-positive bacteria | ||
| − | ==== Usage and biology ==== | + | ===== Usage and biology ===== |
Galacto-N-biose/lacto-N-biose I-binding protein (GL-BP) belongs to the family of ATP-binding cassette-type transporter from Bifidobacterium. The GL-BP are anchored in the membrane of gram-positive bacteria by lipid. | Galacto-N-biose/lacto-N-biose I-binding protein (GL-BP) belongs to the family of ATP-binding cassette-type transporter from Bifidobacterium. The GL-BP are anchored in the membrane of gram-positive bacteria by lipid. | ||
=== BBa_K4004001 === | === BBa_K4004001 === | ||
Revision as of 11:40, 26 September 2022
GLBP-vp7-vp1-amilGFP
GLBP-vp7-vp1-amilGFP
BBa_K4276008
Name: GLBP-vp7-vp1-amilGFP
Base Pairs: 3858 bp
Origin: enterovirus 71, rotaviruses, genome
Properties: tool for monitor the antigens expression
Usage and biology
The researches indicate that the major capsid protein vp1 is the main component of the polyomavirus capsid and the main antigens of EV71. vp1 monomers are typically about 350 amino acids in length and can self-assemble into icosahedral structures consisting of 72 pentamers of 360 vp1 molecules. A capsid protein, vp7, is the main antigens of RV, early studies using sera from hyperimmunized animals in cross-neutralization assays described some different serotypes that were found to infect humans and animals based on the vp7 protein. GLBP is a membrane protein belonging to the family of ATP-binding cassette transport proteins. It is widely expressed in the cell wall of wild-type Bifidobacteria and actively transports specific substances across the cell membrane of all organisms using the energy of adenosine triphosphate (ATP). Antigens expressed in fusion with GLBP can induce mucosal immunity and immune memory. Green fluorescent protein (GFP) is a fluorescent tag that facilitates the detection of vp7-vp1 localization on Bifidobacteria.
Our goal is to produce vp1 and vp7 combined vaccine to protect infants from these two viruses. In addition, the oral method is much more convenient, which can not only improve the vaccination rate, but also does not require professionals to vaccinate, and the consumption of social resources is lower. The plasmid of GLBP-vp7-vp1-amilGFP showed in figure 1.
The components of the plasmid are described as follows:
BBa_K4276000
Name: GLBP
Base Pairs: 1314 bp
Origin: Bifidobacterium, genome
Properties: a protein anchored in the membrane of gram-positive bacteria
Usage and biology
Galacto-N-biose/lacto-N-biose I-binding protein (GL-BP) belongs to the family of ATP-binding cassette-type transporter from Bifidobacterium. The GL-BP are anchored in the membrane of gram-positive bacteria by lipid.
BBa_K4004001
Name: vp1
Base Pairs: 891 bp
Origin: Enterovirus 71 (EV71), genome
Properties: capsid protein and used to be antigen
Usage and biology
The EV71 is a single-stranded RNA virus coated capsid which consists of structural proteins (VP1-VP4). Among the four structural proteins, VP1 is an immunodomaint protein due to distributing in the capsid surface. The VP1 mutations allow to escape the host immune response. Thus, the vaccines were designed based on the VP1 region
BBa_K4276009
Name: vp7
Base Pairs: 883 bp
Origin: Rotaviruses, genome
Properties: a protein anchored in the membrane of gram-positive bacteria
Usage and biology
Rotaviruses are the most common pathogen of gastroenteritis among infants and young children. Rotavirus are members of the Reoviridae family and transmitted through the fecal-oral route. These viruses consist of multilayered, non-enveloped particles with double-stranded RNA. VP4 and VP7 are exposed on the surface of capsid. Thus, these proteins are considered as the antigen to induce the neutralizing antibodies against the Rotavirus. VP7 is a calcium-binding glycoprotein
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 539
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 260
Illegal NgoMIV site found at 700
Illegal NgoMIV site found at 1222
Illegal NgoMIV site found at 3100
Illegal AgeI site found at 2372
Illegal AgeI site found at 3121 - 1000COMPATIBLE WITH RFC[1000]