Difference between revisions of "Part:BBa K4291016"

Revision as of 03:09, 27 September 2022

CueR-CopA-amilGFP

J23119 pro-CueR- rrnB T1 ter- T7Te ter- CopA pro-amilGFP- rrnB T1 ter- T7Te ter

Characterization by Canton_HS

BBa_K4291016

Name: CuR-CopA-amilGFP

Base Pairs: 1703 bp

Origin: E.coli, genome

Properties: Monitor the Cu concentration

Usage and biology

The plasmid was designed to monitor the cooper concentration using the amilGFP intensity. The CueR which belongs to mercury resistance regulator (MerR) family response the mental ions including cooper and silver. Meanwhile, the CueR control the expression of CopA promoter. In the MerR family, there are other metal-responsive metal-regulators including ZntR, CadR, CoaR which response to the heavy metal zinc, cadmium and cobalt, respectively. All of the metal-regulators has the same property that these regulators could balance the heavy metal ions in bacteria via regulating the transcription of heavy-metal-resistance genes.

Figure 1. map of pUC19-CuR-CopA-amilGFP

The components of this plasmid are described as follows:

BBa_K4291012

Name: CueR Base Pairs: 408 bp Origin: E.coli, genome Properties: regulate the cooper efflux

Usage and biology

BBa_K4291012 is an encoding sequence of cooper efflux regulator (CuR) which is sensitive to metal ions such as Cu+, Ag+, Au+. In addition, CuR controls the transcription of copA in response the cooper salt.

BBa_K4291013

Name: CopA promoter Base Pairs: 287 bp Origin: E.coli, genome Properties: Cu+-transporting ATPase pump

Usage and biology

BBa_K4291013 is an encoding sequence copA which is a cooper-transporter P-type ATPase pump. The expression of copA is improved when exposed to metal ions such as silver and cupric ions due to eliminating the CuR effect.

Sequence and Features