Difference between revisions of "Part:BBa K4248007"

 
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==Contribution==
 
==Contribution==
  
LL-37 is expressed widely in human body cells, such as epithelial skin cell,bone marrow cells, and neutrophils. It is responsible for immune defense against bacterial invasion, with study showing its effect of significant reduction in attachment of bacteria and biofilm production
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LL-37 is expressed widely in human body cells, such as epithelial skin cell,bone marrow cells, and neutrophils. It is responsible for immune defense against bacterial invasion, with study showing its effect of significant reduction in attachment of bacteria and biofilm production.
 +
 
 
Because the T7 promoter and T7 RNA polymerase have strong ability in translation and usually be used as protein expression, we choose pET28a-vector and E.coli BL21(DE3), with T7 promoter and T7 RNA polymerase respectively, to express our target protein LL-37. To achieve this, we designed the DNA sequences of LL-37 to be inserted into the XbaI and XhoI sites of the pET-28a(+) vector (Figure 1.), transferred the recombinant plasmid into E.coli BL21(DE3) for protein expression.
 
Because the T7 promoter and T7 RNA polymerase have strong ability in translation and usually be used as protein expression, we choose pET28a-vector and E.coli BL21(DE3), with T7 promoter and T7 RNA polymerase respectively, to express our target protein LL-37. To achieve this, we designed the DNA sequences of LL-37 to be inserted into the XbaI and XhoI sites of the pET-28a(+) vector (Figure 1.), transferred the recombinant plasmid into E.coli BL21(DE3) for protein expression.
 
[[File:T--Shanghai city-- BBa K4248007-figure1.png|500px|thumb|center|Figure 1. The map of recombinant plasmid pET-28a(+)- LL37.]]
 
[[File:T--Shanghai city-- BBa K4248007-figure1.png|500px|thumb|center|Figure 1. The map of recombinant plasmid pET-28a(+)- LL37.]]
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===Usage and Biology===
 
  
 
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Latest revision as of 09:49, 26 September 2022


T7 pro-6His - LL-37- T7 ter

T7 pro-6His - LL-37- T7 ter

Contribution

LL-37 is expressed widely in human body cells, such as epithelial skin cell,bone marrow cells, and neutrophils. It is responsible for immune defense against bacterial invasion, with study showing its effect of significant reduction in attachment of bacteria and biofilm production.

Because the T7 promoter and T7 RNA polymerase have strong ability in translation and usually be used as protein expression, we choose pET28a-vector and E.coli BL21(DE3), with T7 promoter and T7 RNA polymerase respectively, to express our target protein LL-37. To achieve this, we designed the DNA sequences of LL-37 to be inserted into the XbaI and XhoI sites of the pET-28a(+) vector (Figure 1.), transferred the recombinant plasmid into E.coli BL21(DE3) for protein expression.

Figure 1. The map of recombinant plasmid pET-28a(+)- LL37.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 169
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]