Revision as of 08:29, 26 September 2022

mSarlet-I

mSarlet-I

Usage and Biology

Characterized by CAFA_China 2022

• We designed the genetic circuit that contains sulAp (sulA promoter), Amp30E, relE gene (RelE toxin), mScarlet-I and 3WJ-Bro(3WJ-Bro) , and transferred it into DH10B competent cells (The control group did not contain the Amp30E Amplification Device). The gene circuit diagram is shown bellow:

Gene circuit: pSB1C3-sulAp-Amp30E-relE-mScarlet-I-3WJ-Bro

• The relE toxin gene and the mScarlet-I gene were fused to construct a fusion gene, in order to detect the amount of relE protein synthesis. We removed the terminator of relE and fused it to the mScarlet-I gene.
• After bacterial culture and UV induction, we measured the OD600 and fluorescence values under 579/616nm. Then, the fluorescence per OD was calculated.
• The curve of experimental group was higher than that of control group as shown in the following figure, indicating the mScarlet-I correctly displayed the increasing tendency of relE protein synthesis under the effect of Amp30E Amplification Device.

Figure: The mScarlet-I gene was fused with relE gene to detect the amount of relE protein synthesis (579/616nm). Control group: pSB1C3-sulAp-relE-mSarlet-l-3WJxBro; experimental group: pSB1C3-sulAp-Amp30E-relE-mSarlet-l-3WJxBro.

Sequence and Features