Difference between revisions of "Part:BBa K4226000"

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<partinfo>BBa_K4226000 short</partinfo>
 
<partinfo>BBa_K4226000 short</partinfo>
  
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3WJ-Bro is used as a protein- independent reporter system based on a fluorescent RNA aptamer. 3WJ-Bro is applied as a gene marker in creating a system for reporting the presence and expression of target gene, which is relE toxin gene at the transcriptional level in the project of CAFA_China (2022). The 3WJ-Bro can be used to ligate fluorescent aptamers to examine the relE RNA at transcriptional level in Escherichia coli cells, obviating the need for accumulation of foreign proteins.
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3WJ-Bro is used as a protein- independent reporter system based on a fluorescent RNA aptamer. 3WJ-Bro is applied as a gene marker in creating a system for reporting the presence and expression of target genes. The 3WJ-Bro can be used to ligate fluorescent aptamers to examine the relE RNA at transcriptional level in Escherichia coli cells, obviating the need for accumulation of foreign proteins.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:01, 26 September 2022


3WJ-Bro

3WJ-Bro is used as a protein- independent reporter system based on a fluorescent RNA aptamer. 3WJ-Bro is applied as a gene marker in creating a system for reporting the presence and expression of target genes. The 3WJ-Bro can be used to ligate fluorescent aptamers to examine the relE RNA at transcriptional level in Escherichia coli cells, obviating the need for accumulation of foreign proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterized by CAFA_China 2022

  • After bacterial culture and UV induction, we measured the OD600 and fluorescence values under 485/510nm. Then, the fluorescence per OD was calculated.
  • With the lengthening of culture time, the RNA synthesis of relE increased gradually. According to the results, the 3WJ-Bro can be used to ligate fluorescent aptamers to examine the relE RNA synthesis at transcriptional level.
3WJ-Bro was ligated with fluorescent aptamers in order to examine the level of relE RNA synthesis (485/510nm).