Difference between revisions of "Part:BBa K4202007:Experience"
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we transformed it into our chassis-Bacillus subtilis for further protein expression. 50ml shaking flasks with 5ml culture media were introduced to reach an extra-large bacterial population while the normal 12ml shaking bacteria tubes were used as control. | we transformed it into our chassis-Bacillus subtilis for further protein expression. 50ml shaking flasks with 5ml culture media were introduced to reach an extra-large bacterial population while the normal 12ml shaking bacteria tubes were used as control. | ||
From the SDS-PAGE image, we could see a clear strip locating in 120kDa’s area for the 50ml shaking flasks group, revealing the excellent function of Oxygen-limitation induced promotor. However, it seemed that only the first gene (SpaB,120kDa) had expressed under the condition, suggesting that the original sequence didn’t match well to the promotor. | From the SDS-PAGE image, we could see a clear strip locating in 120kDa’s area for the 50ml shaking flasks group, revealing the excellent function of Oxygen-limitation induced promotor. However, it seemed that only the first gene (SpaB,120kDa) had expressed under the condition, suggesting that the original sequence didn’t match well to the promotor. | ||
| − | <div align="center">[[File:GLL-5.png]]</div> | + | <div align="center">[[File:GLL-5.png|200px|thumb|left|alt text]]</div> |
<p><div align="center"><b>Fig 1-1</b>Characterization of Oxygen-limitation induced promotor by SDS-PAGE</div></p> | <p><div align="center"><b>Fig 1-1</b>Characterization of Oxygen-limitation induced promotor by SDS-PAGE</div></p> | ||
Revision as of 03:01, 26 September 2022
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Applications of BBa_K4202007
we transformed it into our chassis-Bacillus subtilis for further protein expression. 50ml shaking flasks with 5ml culture media were introduced to reach an extra-large bacterial population while the normal 12ml shaking bacteria tubes were used as control. From the SDS-PAGE image, we could see a clear strip locating in 120kDa’s area for the 50ml shaking flasks group, revealing the excellent function of Oxygen-limitation induced promotor. However, it seemed that only the first gene (SpaB,120kDa) had expressed under the condition, suggesting that the original sequence didn’t match well to the promotor.
See the [1] 2022 wiki for more details.
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UNIQ125a04531a5490d4-partinfo-00000000-QINU UNIQ125a04531a5490d4-partinfo-00000001-QINU