Difference between revisions of "Part:BBa K4202007"
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<partinfo>BBa_K4202007 parameters</partinfo> | <partinfo>BBa_K4202007 parameters</partinfo> | ||
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| + | ===Applications of BBa_K4202007=== | ||
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| + | we transformed it into our chassis-Bacillus subtilis for further protein expression. 50ml shaking flasks with 5ml culture media were introduced to reach an extra-large bacterial population while the normal 12ml shaking bacteria tubes were used as control. | ||
| + | From the SDS-PAGE image, we could see a clear strip locating in 120kDa’s area for the 50ml shaking flasks group, revealing the excellent function of Oxygen-limitation induced promotor. However, it seemed that only the first gene (SpaB,120kDa) had expressed under the condition, suggesting that the original sequence didn’t match well to the promotor. | ||
| + | <div align="center">[[File:GLL-5.png|600px]]</div> | ||
| + | <p><div align="center"><b>Fig 1-1</b>Characterization of Oxygen-limitation induced promotor by SDS-PAGE</div></p> | ||
| + | |||
| + | See the [https://2022.igem.wiki/zju-china/results] ZJU-China 2022 wiki for more details. | ||
Latest revision as of 03:42, 26 September 2022
PHY-Oxylimited-SpaBCTS
The subtilisin production module is mediated by a hypoxic promoter and functions through a gene cluster composed of SpaBCTS. The population of engineered Bacillus subtilis keeps growing in the cracks, consuming oxygen, and at the same time, the engineered bacteria penetrate deep into the tiny cracks, and the environment lacks oxygen, thus realizing hypoxic conditions, and the subtilisin production module is activated. However we failed to convince the function as the bacteria could only express the first protein under oxygen limitation environment.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 179
Illegal EcoRI site found at 1245
Illegal EcoRI site found at 3776
Illegal XbaI site found at 100
Illegal XbaI site found at 2024
Illegal SpeI site found at 95
Illegal PstI site found at 2674
Illegal PstI site found at 2992 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 179
Illegal EcoRI site found at 1245
Illegal EcoRI site found at 3776
Illegal SpeI site found at 95
Illegal PstI site found at 2674
Illegal PstI site found at 2992 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 179
Illegal EcoRI site found at 1245
Illegal EcoRI site found at 3776
Illegal BamHI site found at 2495
Illegal BamHI site found at 4765 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 179
Illegal EcoRI site found at 1245
Illegal EcoRI site found at 3776
Illegal XbaI site found at 100
Illegal XbaI site found at 2024
Illegal SpeI site found at 95
Illegal PstI site found at 2674
Illegal PstI site found at 2992 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 179
Illegal EcoRI site found at 1245
Illegal EcoRI site found at 3776
Illegal XbaI site found at 100
Illegal XbaI site found at 2024
Illegal SpeI site found at 95
Illegal PstI site found at 2674
Illegal PstI site found at 2992
Illegal AgeI site found at 2309
Illegal AgeI site found at 3317 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 361
Illegal SapI.rc site found at 2409
Illegal SapI.rc site found at 4919
Illegal SapI.rc site found at 6121
Applications of BBa_K4202007
we transformed it into our chassis-Bacillus subtilis for further protein expression. 50ml shaking flasks with 5ml culture media were introduced to reach an extra-large bacterial population while the normal 12ml shaking bacteria tubes were used as control. From the SDS-PAGE image, we could see a clear strip locating in 120kDa’s area for the 50ml shaking flasks group, revealing the excellent function of Oxygen-limitation induced promotor. However, it seemed that only the first gene (SpaB,120kDa) had expressed under the condition, suggesting that the original sequence didn’t match well to the promotor.
See the [1] ZJU-China 2022 wiki for more details.