Difference between revisions of "Part:BBa K4268000:Experience"
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Caption: Colony PCR of five colonies suspected of coating the insert and one colony containing an RFP. The target length is 1573bp. | Caption: Colony PCR of five colonies suspected of coating the insert and one colony containing an RFP. The target length is 1573bp. | ||
Revision as of 23:28, 25 September 2022
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Applications of BBa_K4268000
With the generosity of IDT, SUNY_Oneonta iGEM synthesized the Head-Tail Connector part. This part is RCF1000 compatible and contains SapI sites at either end of the sequence. The part was resuspended, restriction digested, and ligated into PSB1C00. The complex was then transformed into DH5&a cells and was left to grow overnight. After white colony growth was discovered in the transformed cells, five white colonies underwent a Colony PCR to determine if the desired insert was absorbed. Additionally, the selected colonies were restreaked.
Insert gel figure
Caption: Colony PCR of five colonies suspected of coating the insert and one colony containing an RFP. The target length is 1573bp.
The gel indicates that all five colonies are likely to contain the insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part. The confirmed colonies underwent further purification to prepare them for Level 1 Golden Gate Assembly.
User Reviews
UNIQc82e7875ce03f6fe-partinfo-00000000-QINU UNIQc82e7875ce03f6fe-partinfo-00000001-QINU