Difference between revisions of "Part:BBa K4223012"
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5'-UAGACCACCCCAAAAAUGAAGGGGACUAAAACAACUAUACAACCUACUACCUCA-3'<br>
 
5'-UAGACCACCCCAAAAAUGAAGGGGACUAAAACAACUAUACAACCUACUACCUCA-3'<br>
−
crRNA are small guide RNAs that direct the RNAse activity of the Cas13a1 protein against the RNA they hybridize to. Here the crRNA is designed to hybridize to the <partinfo>K4223001</partinfo>,<partinfo>K4223009</partinfo>.
+
crRNA are small guide RNAs that direct the RNAse activity of the Cas13a1 protein against the RNA they hybridize to. Here the crRNA is designed to hybridize to the transcription products of <partinfo>K4223001</partinfo>,<partinfo>K4223009</partinfo> for false positive test.
  
 
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Revision as of 16:51, 25 September 2022


DNA template of crRNA for Cas13a1 in vitro detection system

5'-UAGACCACCCCAAAAAUGAAGGGGACUAAAACAACUAUACAACCUACUACCUCA-3'
crRNA are small guide RNAs that direct the RNAse activity of the Cas13a1 protein against the RNA they hybridize to. Here the crRNA is designed to hybridize to the transcription products of BBa_K4223001,BBa_K4223009 for false positive test.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]