Difference between revisions of "Part:BBa K4202004"
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Biomineralization can be used to deposit calcium carbonate on the surface of microbial cells, filling cracks in stone artifacts. This part is the coding sequence (CDS) of Carbonic anhydrase (CA) encoding a zinc-containing enzyme, &#945;-carbonic anhydrase, which efficiently catalyzes the reversible hydration of CO<sub>2</sub> to rapidly produce bicarbonate (HCO<sub>3</sub><sup>-</sup>) and protons (H<sup>+</sup>).
 
Biomineralization can be used to deposit calcium carbonate on the surface of microbial cells, filling cracks in stone artifacts. This part is the coding sequence (CDS) of Carbonic anhydrase (CA) encoding a zinc-containing enzyme, &#945;-carbonic anhydrase, which efficiently catalyzes the reversible hydration of CO<sub>2</sub> to rapidly produce bicarbonate (HCO<sub>3</sub><sup>-</sup>) and protons (H<sup>+</sup>).
 
<br>
 
<br>
−
H<sub>2</sub>O+CO<sub>2</sub>&#8596;HCO<sub>3</sub><sup>-</sup>+H<sup>+</sup>
+
Chemical reaction equations: H<sub>2</sub>O+CO<sub>2</sub>&#8596;HCO<sub>3</sub><sup>-</sup>+H<sup>+</sup>
 
<br>
 
<br>
−
Bicarbonate (HCO<sub>3</sub><sup>-</sup>) can be transported down the concentration gradient to the outside of the cell. When we provide calcium ions (Ca<sup>2+</sup>) in the extracellular medium with which the bicarbonate can combine to form calcium carbonate precipitates. The calcium carbonate precipitate can accumulate in the microfractures of the stone artifacts, filling the cracks and providing support.
+
Bicarbonate (HCO<sub>3</sub><sup>-</sup>) can be transported down the concentration gradient to the outside of the cell. When we provide calcium ions (Ca<sup>2+</sup>) in the extracellular medium, the bicarbonate can combine to the Ca<sup>2+</sup> to form calcium carbonate precipitates. The calcium carbonate precipitate can accumulate in the tiny cracks of the stone artifacts, filling the cracks and providing support.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:30, 25 September 2022


TSLV-BS-CA

Biomineralization can be used to deposit calcium carbonate on the surface of microbial cells, filling cracks in stone artifacts. This part is the coding sequence (CDS) of Carbonic anhydrase (CA) encoding a zinc-containing enzyme, α-carbonic anhydrase, which efficiently catalyzes the reversible hydration of CO2 to rapidly produce bicarbonate (HCO3-) and protons (H+).
Chemical reaction equations: H2O+CO2↔HCO3-+H+
Bicarbonate (HCO3-) can be transported down the concentration gradient to the outside of the cell. When we provide calcium ions (Ca2+) in the extracellular medium, the bicarbonate can combine to the Ca2+ to form calcium carbonate precipitates. The calcium carbonate precipitate can accumulate in the tiny cracks of the stone artifacts, filling the cracks and providing support.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 540
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 166
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 535