Difference between revisions of "Part:BBa K4140016"

 
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<partinfo>BBa_K4140016 short</partinfo>
 
<partinfo>BBa_K4140016 short</partinfo>
  
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==Part Description==
 
RNA-guided Among its primary targets are single-stranded RNA substrates, Cas12g is a ribonuclease. Comparing it to other Cas12 proteins that have been found so far, CRISPS-Cas12g selectively detects RNA substrates, making it a potentially useful platform for transcriptome editing and diagnostics. While guided RNAs fold into a "F" shape that is primarily identified by the Rec lobes, a bilobed structure of Cas12g displays a tiny NUC2 and REC2 domain. To change the conformation of the REC and NUC lobes and activate Cas12g, target RNA and crRNA guide combine to form a duplex that is inserted into the cavity in the middle of the structure.
 
RNA-guided Among its primary targets are single-stranded RNA substrates, Cas12g is a ribonuclease. Comparing it to other Cas12 proteins that have been found so far, CRISPS-Cas12g selectively detects RNA substrates, making it a potentially useful platform for transcriptome editing and diagnostics. While guided RNAs fold into a "F" shape that is primarily identified by the Rec lobes, a bilobed structure of Cas12g displays a tiny NUC2 and REC2 domain. To change the conformation of the REC and NUC lobes and activate Cas12g, target RNA and crRNA guide combine to form a duplex that is inserted into the cavity in the middle of the structure.
  
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==Usage==
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==Literature Characterization==
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[[File:T--AFCM-EGYPT--CAS2.PNG|thumb|Right|Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs. ]]
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==References==
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 09:56, 28 September 2022


Cas12g


Part Description

RNA-guided Among its primary targets are single-stranded RNA substrates, Cas12g is a ribonuclease. Comparing it to other Cas12 proteins that have been found so far, CRISPS-Cas12g selectively detects RNA substrates, making it a potentially useful platform for transcriptome editing and diagnostics. While guided RNAs fold into a "F" shape that is primarily identified by the Rec lobes, a bilobed structure of Cas12g displays a tiny NUC2 and REC2 domain. To change the conformation of the REC and NUC lobes and activate Cas12g, target RNA and crRNA guide combine to form a duplex that is inserted into the cavity in the middle of the structure.

Usage

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 313
    Illegal PstI site found at 787
    Illegal PstI site found at 1729
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 313
    Illegal PstI site found at 787
    Illegal PstI site found at 1729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1308
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 313
    Illegal PstI site found at 787
    Illegal PstI site found at 1729
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 313
    Illegal PstI site found at 787
    Illegal PstI site found at 1729
    Illegal NgoMIV site found at 1230
  • 1000
    COMPATIBLE WITH RFC[1000]