Difference between revisions of "Part:BBa K4158012:Design"

(→‎Design Notes)
(→‎Design Notes)
 
Line 17: Line 17:
 
   Rv:5'-tcgaattcggatccttagtgatggtg-3'
 
   Rv:5'-tcgaattcggatccttagtgatggtg-3'
  
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3. Restriction and insertion cloning with pET26b(+) and  the PCR productionsand restriction enzymes EcoRI and XbaI
+
3. Restriction and insertion cloning with pET26b(+) and  the PCR production and restriction enzymes EcoRI and XbaI.
  
 
===Source===
 
===Source===

Latest revision as of 02:40, 24 September 2022


SRTF1(E coli Codon Optimized sequence)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal BamHI site found at 532
    Illegal BamHI site found at 683
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
    Illegal AgeI site found at 173
    Illegal AgeI site found at 458
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

"If you want to get the same plasmid that we experimented with, construct the plasmid along this way below.

1. Prepare pET26b(+) cloning vector and this part.

2. Amplitude this part cloning vector with PCR using these Primers below. 

  Fw:5'-ccctctagagcagattctctgatattaacac-3' 

  Rv:5'-tcgaattcggatccttagtgatggtg-3'

3. Restriction and insertion cloning with pET26b(+) and the PCR production and restriction enzymes EcoRI and XbaI.

Source

test

References