Difference between revisions of "Part:BBa K4245113:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
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===Applications of BBa_K4245113===
 
===Applications of BBa_K4245113===
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<b> Lambert_GA 2022 </b>
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This part was experimented with as a part of the composite part, the hsa-mir-451a RCA Padlock Probe (<partinfo>BBa_K4245209</partinfo>).
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<b>RCA with BBa_K4245208</b>
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Rolling Circle Transcription (RCA) was successful with this part. The products of RCA are long DNA strands composed of repeating complementary sequences of the used padlock probe. Therefore, one way in which the success of RCA can be determined is by running the rolling circle products (RCP) on an agarose gel. Since a fluorescent band very close to the wells would indicate the presence of an extremely long DNA strand, our RCP was run on a gel. The result was a really long DNA strand close to the well.
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[[File:File:BBa K4245209_Gel_miR_451a_RCP.jpg|thumb|center|500px|<i>Figure 1. Image of gel ran with miRNA 451a RCP product.</i>]]
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By analyzing the results on the gel, our team concluded that a very long strand of DNA, likely the RCP, was produced. The gel exhibited a fluorescent band of DNA very close to the well, which indicates that a long strand of DNA, greater than 1 kB, was produced due to our reaction (see Fig. 1). As a result, we can infer that the RCA reaction allowed the creation of a really long DNA stand -- our RCP.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 16:55, 11 October 2022

Applications of BBa_K4245113

Lambert_GA 2022 This part was experimented with as a part of the composite part, the hsa-mir-451a RCA Padlock Probe (BBa_K4245209). RCA with BBa_K4245208 Rolling Circle Transcription (RCA) was successful with this part. The products of RCA are long DNA strands composed of repeating complementary sequences of the used padlock probe. Therefore, one way in which the success of RCA can be determined is by running the rolling circle products (RCP) on an agarose gel. Since a fluorescent band very close to the wells would indicate the presence of an extremely long DNA strand, our RCP was run on a gel. The result was a really long DNA strand close to the well.

File:File:BBa K4245209 Gel miR 451a RCP.jpg
Figure 1. Image of gel ran with miRNA 451a RCP product.


By analyzing the results on the gel, our team concluded that a very long strand of DNA, likely the RCP, was produced. The gel exhibited a fluorescent band of DNA very close to the well, which indicates that a long strand of DNA, greater than 1 kB, was produced due to our reaction (see Fig. 1). As a result, we can infer that the RCA reaction allowed the creation of a really long DNA stand -- our RCP.

User Reviews

UNIQbe0d92a4f238d856-partinfo-00000001-QINU UNIQbe0d92a4f238d856-partinfo-00000002-QINU