Difference between revisions of "Part:BBa K4235010:Design"

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<partinfo>BBa_K4235010 SequenceAndFeatures</partinfo>
 
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===Design Notes===
 
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Latest revision as of 23:12, 22 September 2022


mini-attTn7 segment: Tn7R+GmR circuit+Polyhedrin+PROS1+SV40+Tn7L


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2914
    Illegal XbaI site found at 2227
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2914
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2914
    Illegal BglII site found at 37
    Illegal BglII site found at 568
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2914
    Illegal XbaI site found at 2227
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2914
    Illegal XbaI site found at 2227
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The miniatt-Tn7 transposon segment is part of the vector pFastBac BBa_K4235002

References

Bac-to-bac baculovirus expression systems . (n.d.). Retrieved September 22, 2022. http://wolfson.huji.ac.il/expression/bac.pdf