Difference between revisions of "Part:BBa K4235010:Design"
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+ | Bac-to-bac baculovirus expression systems . (n.d.). Retrieved September 22, 2022. http://wolfson.huji.ac.il/expression/bac.pdf |
Revision as of 23:11, 22 September 2022
mini-attTn7 segment: Tn7R+GmR circuit+Polyhedrin+PROS1+SV40+Tn7L
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2914
Illegal XbaI site found at 2227 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2914
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2914
Illegal BglII site found at 37
Illegal BglII site found at 568 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2914
Illegal XbaI site found at 2227 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2914
Illegal XbaI site found at 2227 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
.
Source
The miniatt-Tn7 transposon segment is part of the vector pFastBac BBa_K4235002
References
Bac-to-bac baculovirus expression systems . (n.d.). Retrieved September 22, 2022. http://wolfson.huji.ac.il/expression/bac.pdf