Difference between revisions of "Part:BBa K4115015:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The glgA sRNA is designed to bind the mRNA of glgA to inhibit the expression of glgA.
 
  
 +
The sequence of glgA sRNA is designed as the reverse complementary sequence of 25 base sequences starting from the SD sequences of glgA. And HFQ sequence should be designed in the same plasmid with this sRNA.
  
  
 
===Source===
 
===Source===
  
−
The sequence of glgA sRNA is designed as the reverse complementary sequence of 25 base sequences starting from the SD sequences of glgA, and the fragment we use is artificially synthesized by the company.
+
The glgA gene sequence is gained from the genome of the <i>S.elongatus</i> PCC 6803, and the fragment we use is artificially synthesized by the company.
  
 
===References===
 
===References===

Revision as of 03:38, 4 October 2022


glgA sRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of glgA sRNA is designed as the reverse complementary sequence of 25 base sequences starting from the SD sequences of glgA. And HFQ sequence should be designed in the same plasmid with this sRNA.


Source

The glgA gene sequence is gained from the genome of the S.elongatus PCC 6803, and the fragment we use is artificially synthesized by the company.

References