Difference between revisions of "Part:BBa K4115005:Design"
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===Design Notes===
 
===Design Notes===
−
The sps sRNA is designed to bind the mRNA of sps to inhibit the expression of sps.
+
 
 +
The sequence of sps sRNA is designed as the reverse complementary sequence of 24 base sequences starting from the SD sequences of sps. And HFQ sequence should be designed in the same plasmid with this sRNA.[1]
  
 
===Source===
 
===Source===
  
−
The sequence of sps sRNA is designed as the reverse complementary sequence of 24 base sequences starting from the SD sequences of sps, and the fragment we use is artificially synthesized by the company.
+
The sps gene sequence is gained from the genome of the <i>S.elongatus</i> PCC 6803, and the fragments we use were artificially synthesized by the company.
  
 
===References===
 
===References===
 +
 +
[1] Li, S., Sun, T., Chen, L., Zhang, W. (2021). Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria. In: Basu, C. (eds) Biofuels and Biodiesel. Methods in Molecular Biology, vol 2290. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1323-8_16

Revision as of 03:48, 4 October 2022


sps sRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of sps sRNA is designed as the reverse complementary sequence of 24 base sequences starting from the SD sequences of sps. And HFQ sequence should be designed in the same plasmid with this sRNA.[1]

Source

The sps gene sequence is gained from the genome of the S.elongatus PCC 6803, and the fragments we use were artificially synthesized by the company.

References

[1] Li, S., Sun, T., Chen, L., Zhang, W. (2021). Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria. In: Basu, C. (eds) Biofuels and Biodiesel. Methods in Molecular Biology, vol 2290. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1323-8_16