Difference between revisions of "Part:BBa K4501013:Experience"
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===Applications of BBa_K4501013=== | ===Applications of BBa_K4501013=== | ||
| + | We tested wether the transfection with this part provides exosomes with the ability to be purified by Ni-NTA affinity chromatography (based on the His-tag). For that, we transfected HEK293T cells and collect the supernatant after 24 hours with 0%FBS. Results show that a single shot of transient transfection (by electroporation) is enough to obtain low yield of purified exosomes. | ||
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| + | [[File:CD63.jpg]] | ||
| + | Figure 1. Results on the purification by His-tag mediated affinity chromatogrpahy. (A) Levels of RFP expression on the transfected cells. (B) Yield of exosomes obtained by either ultracentrifuge or affinity chromatography. (C) NTA pattern of the size of particles purified on each condition. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 11:06, 12 October 2022
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K4501013
We tested wether the transfection with this part provides exosomes with the ability to be purified by Ni-NTA affinity chromatography (based on the His-tag). For that, we transfected HEK293T cells and collect the supernatant after 24 hours with 0%FBS. Results show that a single shot of transient transfection (by electroporation) is enough to obtain low yield of purified exosomes.
Figure 1. Results on the purification by His-tag mediated affinity chromatogrpahy. (A) Levels of RFP expression on the transfected cells. (B) Yield of exosomes obtained by either ultracentrifuge or affinity chromatography. (C) NTA pattern of the size of particles purified on each condition.
User Reviews
UNIQfae033f5cb3538b6-partinfo-00000000-QINU UNIQfae033f5cb3538b6-partinfo-00000001-QINU