Difference between revisions of "Part:BBa C0040:Experience"
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| + | <I>Wanglu</I> | ||
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| + | We also met some problem in our construction. It seems that the overexpression of repressor protein has cytotoxicity which made troubles in our process. We just ligated the tetR protein coding sequence with lac promoter, and repeated for many times. However, the sequencing result suggested that there was always either a deletion of the promoter or an unknown insertion in tetR coding sequence in our samples. We speculate that tetR protein has cytotoxicity and burden the E.coli, so the samples were always with mutations that cause of no expression of tetR protein or its inactivation. | ||
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Revision as of 12:38, 16 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_C0040
User Reviews
UNIQ5059c41a6779ec6d-partinfo-00000000-QINU
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Aberdeen_Scotland 2009 |
The transformation, miniprep and the gel (undigested and digested with the EcoRI and SpeI) worked as expected. |
UNIQ5059c41a6779ec6d-partinfo-00000002-QINU UNIQ5059c41a6779ec6d-partinfo-00000003-QINU