Difference between revisions of "Part:BBa K4390110"

 
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<partinfo>BBa_K4390110 short</partinfo>
 
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==Usage and Biology==
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A truncated version of the L2 ribosomal protein from E. coli, for fusion to N-terminal of a protein using JUMP assembly (N-terminal 1-60 and C-terminal 203-273 amino acids of L2 [silica-binding regions of L2 only] 130 amino acids). This tag contains just the N and C-terminal regions of L2 which were shown to have silica binding capacity, allowing the use of a smaller tag without compromising on binding affinity. From literature, the dissociation constant between L2NC silica tag and silica beads is 1.7nM.
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The N-terminal L2NC silica tag is inspired by 2021 Edinburgh OG teams ([[Part:BBa_K3946002|BBa_K3946002]]).
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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==<span class='h3bb'>Sequence and Features</span>==
 
<partinfo>BBa_K4390110 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4390110 SequenceAndFeatures</partinfo>
  
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==Reference==
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Kim S, Joo K, Jo B, Cha H. Stability-Controllable Self-Immobilization of Carbonic Anhydrase Fused with a Silica-Binding Tag onto Diatom Biosilica for Enzymatic CO2 Capture and Utilization. ACS Applied Materials &amp; Interfaces. 2020;12(24):27055-27063.
  
 
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Revision as of 11:31, 12 October 2022


L2NC tag expression (control)

Usage and Biology

A truncated version of the L2 ribosomal protein from E. coli, for fusion to N-terminal of a protein using JUMP assembly (N-terminal 1-60 and C-terminal 203-273 amino acids of L2 [silica-binding regions of L2 only] 130 amino acids). This tag contains just the N and C-terminal regions of L2 which were shown to have silica binding capacity, allowing the use of a smaller tag without compromising on binding affinity. From literature, the dissociation constant between L2NC silica tag and silica beads is 1.7nM. The N-terminal L2NC silica tag is inspired by 2021 Edinburgh OG teams (BBa_K3946002).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 108
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

Kim S, Joo K, Jo B, Cha H. Stability-Controllable Self-Immobilization of Carbonic Anhydrase Fused with a Silica-Binding Tag onto Diatom Biosilica for Enzymatic CO2 Capture and Utilization. ACS Applied Materials & Interfaces. 2020;12(24):27055-27063.