Difference between revisions of "Part:BBa K4115008"

 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K4115008 SequenceAndFeatures</partinfo>
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[[File:CMV-Cas-VP16_Results_Freigem2013.png|900px|]]
  
 
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Revision as of 05:51, 3 October 2022


NifA_downstream

This is a stable sequence located downstream of the nifA sequence in the genome of Azorhizobium caulinodans ORS571. It is used as a one-sided homology arm on the designed plasmid to help recombine knockout of nifA on its original genome and introduce the designed regulatory characterization sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 138
    Illegal BamHI site found at 477
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

CMV-Cas-VP16 Results Freigem2013.png