Difference between revisions of "Part:BBa K4251027"

Latest revision as of 08:57, 13 October 2022

GV248-PDRG1-SiRNA-3

Usage and Biology

GV248-vector is one of the most commonly used siRNA expression vectors, which uses the hU6 promoter to regulate the expression of siRNA. The vector contains an EGFP gene to verify if the plasmid was transfected into the host. We inserted the siRNA3 (BBa_K3251005) which targeted PDRG1, into the AgeI and EcoRI sites and regulated by the hU6 promoter. When expressed in the prokaryotic system, the Amp+ resistance can be used to screen the right colony, when transfected into mammalian cells, the puromycin resistance could be used to screen the transfected cells.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 4265
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2756
Illegal BglII site found at 3595
Illegal BglII site found at 5369
Illegal BglII site found at 5639
Illegal BglII site found at 8329
Illegal BglII site found at 8703
Illegal BamHI site found at 5347
Illegal XhoI site found at 10163
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 185
Illegal NgoMIV site found at 246
Illegal NgoMIV site found at 360
Illegal NgoMIV site found at 8083
Illegal NgoMIV site found at 9282
Illegal AgeI site found at 6812
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 805
Illegal SapI.rc site found at 4229

@@ Line 5: / Line 5: @@
 GV248-PDRG1-SiRNA-3
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+GV248-vector is one of the most commonly used siRNA expression vectors, which uses the hU6 promoter to regulate the expression of siRNA. The vector contains an EGFP gene to verify if the plasmid was transfected into the host. We inserted the siRNA3 (BBa_K3251005) which targeted PDRG1, into the AgeI and EcoRI sites and regulated by the hU6 promoter. When expressed in the prokaryotic system, the Amp+ resistance can be used to screen the right colony, when transfected into mammalian cells, the puromycin resistance could be used to screen the transfected cells.
 <!-- -->