Difference between revisions of "Part:BBa K4324000"

Revision as of 02:02, 28 September 2022

lacPO-RBS-XR-T1

This part allows E. coli to express xylose reductase (XR).

XR is an enzyme that serves as a catalyst for the conversion of xylose into xylitol, and vice versa, according to the following chemical equation:

D-xylose + NAD(P)H + H+ ⇌ xylitol + NAD(P)+

In S. stipitis yeast cells, XR forms the first process in the XR-XDH pathway, which converts xylose into xylulose via xylitol. Xylulose is then converted into xylulose-5-phosphate (X5P) for further metabolism in the pentose phosphate pathway.

E. coli do not exhibit the XR-XDH pathway, instead having an XI pathway which directly converts xylose into xylulose. Hence, together with xylitol dehydrogenase (XDH) which can convert xylitol to xylulose, XR presents an alternate xylose metabolism pathway for E. coli.

Furthermore, as the reaction from xylose to xylitol is reversible, this part enables E. coli to utilise xylitol as an energy source through its conversion to xylose, which then follows the XI pathway.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 827

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4324000 short</partinfo>
-test
+This part allows E. coli to express xylose reductase (XR).
+XR is an enzyme that serves as a catalyst for the conversion of xylose into xylitol, and vice versa, according to the following chemical equation:
+D-xylose + NAD(P)H + H+ ⇌ xylitol + NAD(P)+
+In S. stipitis yeast cells, XR forms the first process in the XR-XDH pathway, which converts xylose into xylulose via xylitol. Xylulose is then converted into xylulose-5-phosphate (X5P) for further metabolism in the pentose phosphate pathway.
+E. coli do not exhibit the XR-XDH pathway, instead having an XI pathway which directly converts xylose into xylulose. Hence, together with xylitol dehydrogenase (XDH) which can convert xylitol to xylulose, XR presents an alternate xylose metabolism pathway for E. coli.
+Furthermore, as the reaction from xylose to xylitol is reversible, this part enables E. coli to utilise xylitol as an energy source through its conversion to xylose, which then follows the XI pathway.
 <!-- Add more about the biology of this part here