Difference between revisions of "Part:BBa K4361106:Design"
 
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===Design Notes===
 
===Design Notes===
−
TBD
+
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Latest revision as of 15:41, 12 October 2022


pET-11a with BlcR with 6xHis-tag and TEV protease cleavage site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
    Illegal XbaI site found at 5594
    Illegal PstI site found at 1068
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
    Illegal NheI site found at 5639
    Illegal NheI site found at 6446
    Illegal PstI site found at 1068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 5528
    Illegal BamHI site found at 5672
    Illegal BamHI site found at 6581
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
    Illegal XbaI site found at 5594
    Illegal PstI site found at 1068
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
    Illegal XbaI site found at 5594
    Illegal PstI site found at 1068
    Illegal NgoMIV site found at 3394
    Illegal NgoMIV site found at 3748
    Illegal NgoMIV site found at 3908
    Illegal NgoMIV site found at 5496
    Illegal NgoMIV site found at 5830
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1242
    Illegal SapI.rc site found at 2324
    Illegal SapI.rc site found at 6341


Design Notes

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Source

-Combination of Part:BBa_K4361105 and Part:BBa_K4361103.

References